Serum glutamic oxalacetic transaminase assay using the sequential multiple analyzer (SMA 12-30).
نویسندگان
چکیده
The SMA-12/30 method for serum glutamic oxalacetic transaminase assay gives higher values than are obtained with a reference spectrophotometric method for someserums.A modification of the manifold is made to permit the running of serum blanks. Blank corrections on 466 serum samples indicate that dialysis does not eliminate the need for the blank determination. Acetoacetate interferes in the SMA12/30 method. The possibilityof other interfering substanceshas not been ruled out.
منابع مشابه
The significance of the serum glutamic oxalacetic transaminase activity following acute myocardial infarction.
The serum glutamic oxalacetic transamninase rose two to 20 times normal in 74 of 75 patients who had acute transmural myocardial infarction. The height of the enzymatic activity was roughly proportional to the size of the infarct. Serum glutamic oxalacetic transaminase is unaffecte:d by angina pectoris, coronary insufficiency, heart failure, or digitalis in the absence of active heart cell inju...
متن کاملAutomated Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminase.
The determination of serum glutamic oxalacetic and glutamic pyruvic transaminase has been simplified by the use of automated analysis. The flow diagrams, concentration of reagents, and calculation of the color unit in terms of the Karmen unit are shown for 2,4-dinitrophenylhydrazine and 6-benzamido-4-methoxy-m -toluidine diazonium chloride as the reacting dyes. Advantages of both methods are co...
متن کاملElectrophoresis of Glutamic Oxalacetic Transaminase in Serum, Beef Heart, and Liver Homogenates on Cellulose Acetate.
‘THE RECENT DEVELOPMENT of a new colorimetric procedure (TransAc*), for the determination of serum glutamic oxalacetic transaminase utilizing the diazonium salt, 6-benzamido-4-methoxy-m-toluidine diazonium chloride, as the color developer with a specificity for oxalacetic acid, prompted the following experiments. Determination of glutamic oxalacetic transaminase activities were performed accord...
متن کاملTechnical improvements in measurement of L -aspartate:2-oxoglutarate aminotransferase activity in serum by diazonium salt coupling.
3. Jacobs, W. L. W., Gamrna-glutamyl transpeptidase in disease of the liver, cardiovascularsystem and diabetesmellitus.Clin. Chim. Acta 38,419(1972). 4. Karmen, A., A note on the spectrophotometric assay of glutamic oxalacetic transaminase in human blood serum. J. Clin. Invest. 34, 131 (1955). 5. Wroblewski, F., and La Due, J. S., Serum glutamic pyruvic transaminase in cardiac and hepatic disea...
متن کاملDetermination of glutamic oxalacetic transaminase activity by coupling of oxalacetate with diazonium salts.
A method for measuring serum glutamic oxalacetic transaminase activity is described, in which substrate concentrations are more nearly optimal than in previous colonmetric methods. By coupling the diazonium salt at a pH of 4.2, interference from a-oxoglutarate is negligible. Oxalacetate is produced at a linear rate to 180 l.U. Results correlate well with those obtained by a reference spectropho...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 15 8 شماره
صفحات -
تاریخ انتشار 1969